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[Expression of Nogo-A and Nogo receptor in neonatal rats visual system during development]
Yan Huo1, Rong-di Yuan, Jian Ye
1Department of Ophthalmology, Daping Hospital, the Third Military Medical University, Chongqing 400042, China.
Objective:
To study the expression of Nogo-A and Nogo receptor (NgR) in the development of neonatal rats retinal and visual cortex and their effects on the development of central nervous system (CNS).
Methods:
Immunohistochemical staining method was used to detect the expression of Nogo-A and NgR in neonatal rats retinal and visual cortex at 1, 3, 7, 14, 28, 56 days after birth. One-way Anova was used to analyze the results of Western blot analysis of Nogo-A and NgR in neonatal rats visual cortex.
Results:
Nogo-A and NgR was found to express in neonatal rats retinal and visual cortex at the developing stage. In 1, 3, 7, 14, 28, 56 days, the IDV ratio of Nogo-A was 0.852 ± 0.026, 0.917 ± 0.024, 0.810 ± 0.028, 0.417 ± 0.053, 0.258 ± 0.029, 0.298 ± 0.054 respectively and the IDV ratio of NgR was 0.070 ± 0.014, 0.185 ± 0.035, 0.678 ± 0.046, 0.705 ± 0.021, 1.210 ± 0.057, 1.140 ± 0.0420 respectively by Western blot analysis. The expression of Nogo-A was decreased (F = 376.56, P = 0.000) and the expression of NgR was increased (F = 888.26, P = 0.000) during postnatal development.
Conclusion:
Nogo-A and NgR is closely related to the CNS growth and development.
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