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Updated: Jun 3, 2026

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Corneal Tissue Engineering: An In Vitro Model of the Stromal-nerve Interactions of the Human Cornea

Published on: January 24, 2018

[Cell sheet technology and its applications in corneal tissue engineering].

Qiao-Ling Wei¹, Jian-Jiang Xu

¹Department of Ophthalmology, Eye Ear Nose and Throat Hospital of Fudan University, Shanghai 200031, China.

[Zhonghua Yan Ke Za Zhi] Chinese Journal of Ophthalmology

|March 23, 2011

Summary

This summary is machine-generated.

Cell sheet technology utilizes temperature-responsive polymers to harvest intact cell sheets with extracellular matrix for tissue engineering. This method overcomes immune rejection and donor shortages in corneal reconstruction.

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Last Updated: Jun 3, 2026

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Area of Science:

Biomaterials Science
Tissue Engineering
Polymer Chemistry

Context:

Cell sheet technology (CST) leverages poly(N-isopropylacrylamide) (PNIPAAm) and its temperature-dependent hydration properties.
PNIPAAm undergoes reversible hydration/dehydration across its lower critical solution temperature (LCST) of 32°C.
Lowering temperature to 20°C induces a hydrophobic-to-hydrophilic transition, enabling harvest of intact cell sheets with extracellular matrix (ECM).

Purpose:

To review the preparation of temperature-responsive surfaces for cell sheet technology.
To analyze factors influencing cell adhesion and proliferation on these surfaces.
To summarize clinical applications and animal studies of CST in corneal tissue engineering.

Summary:

CST allows harvesting of cell sheets with intact ECM by utilizing temperature-responsive polymers like PNIPAAm.
Harvested cell sheets maintain viability and function, enabling scaffold-free transplantation and 3D tissue construction.
Surface preparation, polymer characteristics, and additives critically impact cell behavior and sheet quality.

Impact:

CST offers a promising solution for corneal reconstruction by using autologous/allogeneic cells, mitigating immune rejection and donor scarcity.
Successful clinical applications include limbal epithelium and oral mucosal epithelium grafts for ocular surface restoration.
This technology has significant potential for regenerative medicine and advanced tissue engineering applications.