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Updated: Jun 3, 2026

Pulse-chase Analysis of N-linked Sugar Chains from Glycoproteins in Mammalian Cells
Published on: April 27, 2010
CGR3: a Golgi-localized protein influencing homogalacturonan methylesterification
Michael A Held1, Evan Be, Starla Zemelis
1Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.
Abstract:
Plant cell walls are complex structures that offer structural and mechanical support to plant cells and are ultimately responsible for plant architecture and form. Pectins are a large group of complex polysaccharides of the plant cell wall that are made in the Golgi and secreted to the wall. The methylesterification of pectins is believed to be an important factor for the dynamic properties of the cell wall. Here, we report on a protein of unknown function discovered using an extensive proteomics analysis of cotton Golgi. Through bioinformatic analyses, we identified the ortholog of such protein, here named cotton Golgi-related 3 (CGR3) in Arabidopsis and found that it shares conserved residues with S-adenosylmethionine methyltransferases. We established that CGR3 is localized at the Golgi apparatus and that the expression of the CGR3 gene is correlated with that of several cell wall biosynthetic genes, suggesting a possible role of the protein in pectin modifications. Consistent with this hypothesis, immunofluorescence microscopy with antibodies for homogalacturonan pectins (HG) indicated that the cell walls of cgr3 knockout mutants and plants overexpressing CGR3 are decreased and increased in HG methylesterification, respectively. Our results suggest that CGR3 plays a role in the methylesterification of homogalacturonan in Arabidopsis.
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