Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
Responses to Heat and Cold Stress02:45

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term outcomes and quantitative comparison of mucosal repair using skin grafting versus polyglycolic acid sheet application for cT1-2 oral cancer defect.

Acta oto-laryngologica·2026
Same author

Methylglyoxal Promotes RBC-Driven Procoagulant Activity and Venous Thrombosis under Diabetes-Relevant Conditions.

Thrombosis and haemostasis·2026
Same author

Engineered antibodies bypass bacterial immune evasion to drive complement-mediated protection against lethal infections.

Science translational medicine·2026
Same author

Genetic Variation and Spatial Genetic Structure of <i>Eleocharis ussuriensis</i> Zinserl. in South Korea: Implications for Ecological Monitoring and Resource Management.

Genes·2026
Same author

Clinical Impact of Rapid Recurrence in Oral Tongue Squamous Cell Carcinoma and a Nomogram for Early High-Risk Identification.

Clinical and experimental otorhinolaryngology·2026
Same author

An Engineered Abscisic Acid Receptor Enhances ABA Signaling and Improves Abiotic Stress Tolerance in Rice.

Plant, cell & environment·2026
Same journal

<i>In vitro</i> antibacterial activity of gepotidacin in combination with other antimicrobial agents against <i>Neisseria gonorrhoeae</i> isolates.

Antimicrobial agents and chemotherapy·2026
Same journal

Development of domain-specific probes of <i>Plasmodium falciparum</i> heat shock protein 70-1.

Antimicrobial agents and chemotherapy·2026
Same journal

Addressing therapeutic options for KPC-3-producing ST307-<i>Klebsiella pneumoniae</i>: insights from <i>in vitro</i> evolution and mutant prevention strategies.

Antimicrobial agents and chemotherapy·2026
Same journal

Indole-based hybrids target both asexual parasites and gametocytes of <i>Plasmodium falciparum</i> and synergize with lumefantrine.

Antimicrobial agents and chemotherapy·2026
Same journal

Lineage-specific loss of the type VI secretion system in <i>Acinetobacter baumannii</i> ST19 is associated with reduced accessory genome content.

Antimicrobial agents and chemotherapy·2026
Same journal

Genomic diversity and topical antimicrobial resistance in <i>Staphylococcus aureus</i> from clinical and carriage populations in the New York-New Jersey region.

Antimicrobial agents and chemotherapy·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain
07:57

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

Published on: July 11, 2016

Decrease in penicillin susceptibility due to heat shock protein ClpL in Streptococcus pneumoniae.

Thao Dang-Hien Tran1, Hyog-Young Kwon, Eun-Hye Kim

  • 1School of Pharmacy, Sungkyunkwan University, Suwon 440-746, South Korea.

Antimicrobial Agents and Chemotherapy
|March 23, 2011
PubMed
Summary
This summary is machine-generated.

Heat shock protein ClpL modulates penicillin susceptibility in Streptococcus pneumoniae by interacting with cell wall synthesis protein PBP2x. Loss of ClpL increases susceptibility, while overexpression enhances resistance, highlighting ClpL as a potential therapeutic target.

More Related Videos

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70
07:14

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70

Published on: March 8, 2024

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
13:52

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

Published on: July 9, 2013

Related Experiment Videos

Last Updated: Jun 3, 2026

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain
07:57

Prevention of Heat Stress Adverse Effects in Rats by Bacillus subtilis Strain

Published on: July 11, 2016

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70
07:14

Escherichia coli -Based Complementation Assay to Study the Chaperone Function of Heat Shock Protein 70

Published on: March 8, 2024

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
13:52

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae

Published on: July 9, 2013

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biochemistry

Background:

  • Antibiotic resistance poses a significant global health threat, necessitating research into bacterial tolerance mechanisms.
  • Streptococcus pneumoniae is a major human pathogen, and understanding its antibiotic tolerance is crucial for effective treatment.

Purpose of the Study:

  • To investigate the role of the heat shock protein ClpL in modulating penicillin susceptibility in Streptococcus pneumoniae.
  • To elucidate the molecular mechanisms by which ClpL affects cell wall synthesis and antibiotic tolerance.

Main Methods:

  • Generation and analysis of ClpL knockout and overexpressing strains of S. pneumoniae.
  • Assessment of penicillin susceptibility, cell wall thickness, and cell wall synthesis gene expression (pbp2x).
  • Co-immunoprecipitation and His tag pulldown assays to confirm protein-protein interactions between ClpL and PBP2x.

Main Results:

  • Mutants lacking ClpL exhibited decreased penicillin resistance and thinner cell walls, while ClpL-overexpressing strains showed increased resistance and thicker cell walls.
  • Heat shock induced ClpL-dependent upregulation of pbp2x expression, correlating with altered penicillin susceptibility.
  • ClpL was localized to the cell wall upon heat shock and was found to interact with PBP2x, facilitating its translocation.

Conclusions:

  • ClpL plays a critical role in regulating penicillin susceptibility in S. pneumoniae by stabilizing pbp2x expression and interacting with PBP2x.
  • ClpL's modulation of cell wall synthesis contributes to antibiotic tolerance, presenting a potential target for novel therapeutic strategies.