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Related Concept Videos

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Updated: Jun 3, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

Northern blotting.

J W Pollard¹, C R Perry, C F Thurston

¹MRC Group in Human Genetic Diseases, Department of Biochemistry, King's College, University of London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 23, 2011

Summary

This summary is machine-generated.

Northern blotting detects specific RNA sequences using hybridization probes. This method separates RNA by electrophoresis, immobilizes it on a filter, and identifies target sequences by probe binding, determining size and relative amounts.

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Published on: October 14, 2010

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Last Updated: Jun 3, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

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Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

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Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Area of Science:

Molecular Biology
Genetics

Background:

Northern blotting is a widely used technique in molecular biology.
It enables the detection of specific ribonucleic acid (RNA) sequences within complex samples.

Purpose of the Study:

To describe the methodology and applications of Northern blotting.
To explain how Northern blotting identifies and quantifies specific RNA molecules.

Main Methods:

RNA samples are separated using denaturing gel electrophoresis.
Separated RNA is transferred (blotted) onto a solid membrane filter.
Specific RNA sequences are detected by hybridization with labeled complementary nucleic acid probes.

Main Results:

The technique allows for the specific detection of RNA sequences homologous to the probe.
The size of the detected RNA species can be determined.
Relative amounts of specific RNA can be compared across different samples.

Conclusions:

Northern blotting is a powerful tool for analyzing RNA expression.
It provides valuable information on RNA size and abundance.
The method is crucial for understanding gene expression patterns.