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Related Concept Videos

Translation01:31

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Proteins are called the...
Translation01:31

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation01:31

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation01:31

Translation

Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...
Termination of Translation01:44

Termination of Translation

The large ribosomal subunit has several important structures essential to translation. These include the peptidyl transferase center (PTC) - which is the site where the peptide bond is formed - and a large, internal, water-filled tube through which the nascent polypeptide moves. This latter structure is called the Peptide Exit Tunnel, and it begins at the PTC and spans the body of the large ribosomal subunit. During translation, as the nascent polypeptide chain is synthesized, it passes through...

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Related Experiment Video

Updated: Jun 3, 2026

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Hybrid-release translation.

A Slater1

  • 1School of Life Sciences, Leicester Polytechnic, Leicester, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 23, 2011
PubMed
Summary
This summary is machine-generated.

Hybrid-release translation identifies specific messenger RNA (mRNA) using cloned DNA on a solid support. This method isolates and translates complementary mRNA in vitro for recombinant DNA research.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Genetics

Background:

  • Recombinant DNA research requires identifying specific mRNA from cloned DNA sequences.
  • Characterizing cloned DNA involves hybridization with mRNA.
  • Two methods exist: hybrid-arrest translation and hybrid-release translation.

Purpose of the Study:

  • To describe the hybrid-release translation method for isolating and identifying specific mRNA.
  • To provide a technique for characterizing cloned DNA sequences.

Main Methods:

  • Cloned DNA is immobilized on a solid support.
  • The solid support with DNA is used to hybridize with an mRNA population.
  • Complementary mRNA is isolated by elution.
  • Isolated mRNA is translated in vitro.

Main Results:

  • Successful isolation of specific mRNA complementary to cloned DNA.
  • In vitro translation of the isolated mRNA to identify the encoded protein.

Conclusions:

  • Hybrid-release translation is an effective method for identifying mRNA encoded by cloned DNA.
  • This technique is valuable for characterizing DNA fragments in molecular biology.