Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Plant Tissue Culture

Plant Tissue Culture

Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.

Transcription

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...

Transcription

Transcription

Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...

Methods of Nuclear Reprogramming

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ribonucleic acid synthesis by chromatin isolated from Phaseolus aureus Roxb. : The effect of endogenous ribonuclease.

Planta·2014

Same author

Characterization of ribonucleic acid synthesis by nuclei isolated from Zea mays.

Planta·2014

Same author

Random amplified polymorphic DNA (RAPD) markers for genetic analysis inAllium.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

Isolation of plant nuclei.

Methods in molecular biology (Clifton, N.J.)·2011

Same author

The extraction of total RNA by the detergent and phenol method.

Methods in molecular biology (Clifton, N.J.)·2011

Same author

The purification of poly(a)-containing RNA by affinity chromatography.

Methods in molecular biology (Clifton, N.J.)·2011

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

In vitro transcription in plant nuclei.

R D Henfrey¹, R J Slater

¹Division of Biology and Environmental Sciences, The Hatfield Polytechnic, Hertfordshire, UK.

Methods in Molecular Biology (Clifton, N.J.)

|March 23, 2011

Summary

This study details a method for measuring plant nuclear RNA polymerase activity. Researchers can quantify total and specific RNA polymerase I, II, and III activities using radiolabeled precursors and alpha-amanitin.

Area of Science:

Molecular Biology
Plant Science
Biochemistry

Background:

Isolated plant nuclei are crucial for studying the transcription machinery.
Quantifying RNA polymerase activity is essential for understanding gene expression regulation.

Purpose of the Study:

To describe a method for measuring total and individual RNA polymerase activities in isolated plant nuclei.
To enable fundamental research on plant transcription processes.

Main Methods:

Incubation of isolated plant nuclei with radiolabeled RNA synthesis substrates (ATP, GTP, CTP, UTP).
Differential inhibition of RNA polymerases using varying concentrations of α-amanitin.
Detection of transcription via incorporation of radioactivity into acid-insoluble material.

More Related Videos

In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

In Situ Hybridization for the Precise Localization of Transcripts in Plants

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

Published on: September 20, 2016

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

Precipitation, filtration, and liquid scintillation counting of RNA products.

Main Results:

The assay allows for the measurement of total RNA polymerase activity.
Specific activities of RNA polymerase I, II, and III can be distinguished by α-amanitin sensitivity.
Quantification of newly synthesized RNA is achieved through radioactivity detection.

Conclusions:

This method provides a robust approach for assessing plant nuclear RNA polymerase activity.
It facilitates detailed investigations into the plant transcription apparatus and gene regulation.