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Random amplified polymorphic DNA (RAPD) markers for genetic analysis inAllium.

S E Wilkie1, P G Isaac, R J Slater

  • 1Biosciences Department, University of Hertfordshire, College Lane, AL10 9AB, Hatfield, Herts, England.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 7, 2013
PubMed
Summary
This summary is machine-generated.

Randomly Amplified Polymorphic DNA (RAPD) analysis reveals genetic diversity within the Allium genus. This molecular marker technique is suitable for onion genetic studies and phylogenetic investigations.

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Bioinformatics

Background:

  • The Allium genus, including economically important crops like onion (Allium cepa), requires detailed genetic characterization.
  • Understanding genetic diversity and phylogenetic relationships is crucial for crop improvement and taxonomic classification.
  • Randomly Amplified Polymorphic DNA (RAPD) analysis offers a potential method for assessing genetic variability in plants.

Purpose of the Study:

  • To evaluate the effectiveness of RAPD analysis for assessing genetic polymorphism in onion and related Allium species.
  • To investigate the phylogenetic relationships among different Allium cultivars and species.
  • To identify suitable RAPD markers for genetic mapping in onion.

Main Methods:

  • RAPD analysis was performed on seven Allium cepa cultivars (including shallot) and five other Allium species using 20 random 10-mer primers.
  • Scorable polymorphisms were identified, and genetic distances were calculated between cultivars.
  • Cluster analysis was employed to generate a dendrogram illustrating phylogenetic relationships.

Main Results:

  • Seven out of twenty primers yielded scorable polymorphisms within Allium cepa cultivars, indicating their potential for genetic mapping.
  • Significant variations in banding profiles were observed across different Allium species, suitable for systematic studies.
  • Cluster analysis broadly supported existing classifications but suggested Allium roylei is more closely related to Allium cepa than currently recognized.

Conclusions:

  • RAPD analysis is a valid and effective method for assessing genetic diversity and phylogenetic relationships within the Allium genus.
  • The study identified polymorphic markers for potential use in onion genetic mapping.
  • The findings challenge the current taxonomic placement of Allium roylei, suggesting a closer relationship to onion.