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Chemiluminescence detectors for liquid chromatography
Jacqui L Adcock1, Jessica M Terry, Colin J Barrow
1School of Life and Environmental Sciences, Deakin University, Geelong, Victoria 3217, Australia.
Drug Testing and Analysis
|March 23, 2011
Summary
This tutorial details building chemiluminescence detectors for High-Performance Liquid Chromatography (HPLC). It covers essential components, construction, and data acquisition, plus reviews commercial HPLC detectors.
Area of Science:
- Analytical Chemistry
- Instrumentation Science
Background:
- High-Performance Liquid Chromatography (HPLC) is a crucial separation technique.
- Chemiluminescence (CL) detection offers high sensitivity for various analytes.
- Developing robust and accessible CL detectors for HPLC is essential for advanced analytical applications.
Purpose of the Study:
- To provide a comprehensive guide on constructing custom chemiluminescence detectors for HPLC.
- To detail the necessary components, including reagent delivery, flow cells, and photomultiplier tubes.
- To review current state-of-the-art commercial chemiluminescence detectors for HPLC and flow analysis.
Main Methods:
- Description of the construction of a chemiluminescence detector.
- Inclusion of components: reagent delivery system, coiled-tubing flow cell, photomultiplier tube, and detector housing.
- Discussion of data acquisition options and commercial detector systems.
Main Results:
- A functional chemiluminescence detector for HPLC can be constructed using described components.
- The tutorial outlines practical considerations for building and optimizing such detectors.
- Commercial alternatives are presented for comparison and selection.
Conclusions:
- Custom chemiluminescence detectors for HPLC can be built with accessible components.
- Understanding detector construction enhances analytical method development.
- The review provides valuable insights for selecting appropriate HPLC detection systems.
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