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Updated: Jun 3, 2026

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
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Published on: April 3, 2014

Tetraspanin CD9 in cell migration.

Dale Powner1, Petra M Kopp, Susan J Monkley

  • 1School of Cancer Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

Biochemical Society Transactions
|March 25, 2011
PubMed
Summary
This summary is machine-generated.

Tetraspanin CD9 deficiency impairs talin1 localization to focal adhesions, a key regulator of integrin activation. This leads to increased breast cancer cell motility, revealing a novel CD9-dependent mechanism in cell migration.

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Last Updated: Jun 3, 2026

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
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Published on: April 3, 2014

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Published on: March 20, 2026

In vitro Cell Migration and Invasion Assays
09:55

In vitro Cell Migration and Invasion Assays

Published on: June 1, 2014

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Tetraspanin CD9 interacts with integrin adhesion receptors, influencing cell migration and invasion.
  • CD9's role in cell migration is linked to signaling pathways like FAK, PI3K, p38 MAPK, and JNK.
  • Integrin activation is crucial for cell adhesion and migration, regulated by proteins like talin1.

Purpose of the Study:

  • To elucidate a novel mechanism by which CD9 regulates integrin-dependent cell migration.
  • To investigate the specific role of CD9 in the localization of talin1 to focal adhesions.
  • To determine the impact of CD9 deficiency on breast cancer cell motility.

Main Methods:

  • Analysis of CD9-deficient cells.
  • Immunofluorescence microscopy to assess talin1 localization at focal adhesions.
  • Cell motility assays to quantify breast cancer cell migration.

Main Results:

  • CD9 deficiency results in impaired localization of talin1 to focal adhesions.
  • Loss of CD9 correlates with increased motility of breast cancer cells.
  • This suggests CD9 plays a critical role in regulating talin1 dynamics at focal adhesions.

Conclusions:

  • CD9 specifically controls the localization of talin1 to focal adhesions.
  • CD9-deficiency disrupts normal talin1 localization, leading to enhanced breast cancer cell migration.
  • This finding uncovers a new mechanism by which CD9 influences cell adhesion and motility.