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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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A Polyaniline-based Sensor of Nucleic Acids
07:58

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Published on: November 1, 2016

Coordination polymer nanobelts for nucleic acid detection.

Yonglan Luo1, Fang Liao, Wenbo Lu

  • 1Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, School of Chemistry and Chemical Industry, China West Normal University, Nanchong, Sichuan, People's Republic of China.

Nanotechnology
|March 25, 2011
PubMed
Summary

This study introduces coordination polymer nanobelts (CPNBs) for rapid nucleic acid detection. The CPNBs offer sensitive and selective fluorescent sensing of DNA, even with single-base mismatches.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biochemistry

Background:

  • Coordination polymers offer versatile structural and functional properties.
  • Developing rapid and sensitive nucleic acid detection methods is crucial for diagnostics.
  • Fluorescent sensing platforms provide high sensitivity for biomolecule detection.

Purpose of the Study:

  • To rapidly prepare coordination polymer nanobelts (CPNBs) on a large scale.
  • To explore the application of CPNBs as a fluorescent sensing platform for nucleic acid detection.
  • To investigate the sensitivity and specificity of CPNB-based DNA detection.

Main Methods:

  • Rapid, large-scale synthesis of CPNBs via room-temperature mixing of AgNO(3) and 4,4'-bipyridine solutions.
  • Utilizing π-π stacking interactions between CPNBs and single-stranded DNA (ssDNA) for fluorescence quenching.
  • Observing fluorescence recovery upon hybridization of target DNA with the probe, preventing adsorption.

Main Results:

  • CPNBs were synthesized rapidly and on a large scale.
  • ssDNA adsorption onto CPNBs resulted in 66% fluorescence quenching.
  • Target DNA presence led to significant fluorescence enhancement (73% recovery), indicating successful detection.
  • The system demonstrated high sensitivity, detecting targets as low as 5 nM.
  • Outstanding discrimination ability was achieved, distinguishing even single-base mismatches.

Conclusions:

  • CPNBs serve as an effective platform for sensitive and selective fluorescent detection of nucleic acids.
  • The developed method offers rapid, large-scale synthesis and robust detection capabilities.
  • This approach holds promise for advanced diagnostic tools and molecular sensing applications.