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Updated: Jun 3, 2026

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders
Published on: February 9, 2022
Adipogenic differentiation of human mesenchymal stem cells
1Laboratory for Stem Cell Research, Aalborg University, Aalborg, Denmark. trinef@hst.aau.dk
Abstract:
Mesenchymal stem cells have the capability to differentiate into a number of cell types including adipocytes. The adipocytic phenotype is characterized by intracellular accumulation of lipid droplets as well as transcription of adipocyte-specific genes. This paper details a basic protocol for adipogenic induction of bone marrow and adipose tissue-derived stem cells, as well as protocols for staining lipid accumulation and the transcriptional analysis of PPAR-γ and aP2 by real-time RT-PCR.

