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Updated: Jun 3, 2026

Purification, Expansion, and Flow Cytometry-Based Phenotyping of Mouse Derived Bone Marrow Mesenchymal Stem Cells
Published on: July 11, 2025
Phenotypic analysis and differentiation of murine mesenchymal stem cells
Lindolfo da Silva Meirelles1, Dimas Tadeu Covas
1National Institute of Science and Technology for Stem Cells and Cell Therapy, Centro Regional de Hemoterapia de Ribeirão Preto-HCFMRP/Universidade de São Paulo, Ribeirão Preto, SP, Brazil. lindolfo_meirelles@hotmail.com
Abstract:
Mesenchymal stem cells (MSCs) hold great promise as therapeutic tools to treat different types of disease and the use of preclinical animal models is mandatory for the development of novel MSC-based therapies. The mouse is one of the most important species used for preclinical experiments and, by extension, so is the isolation and characterization of murine MSCs. This chapter presents methods for the phenotypic analysis of cultured murine MSCs.

