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Published on: July 11, 2013
Boronic Acid matrices for the affinity purification of glycoproteins and enzymes
1Department of Chemistry, New Mexico State University, Las Cruces, NM.
Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
Abstract:
Boronic acids immobilized on insoluble matrices have been used for over a decade to purify proteins and enzymes (1,2). Boronic acid matrices have been used to purify: 1. Glycoproteins, 2. Enzymes for which a boronic acid moiety is an inhibitor, and 3. Enzymes that bind to specific dial-containing cofactors (such as UTP or NADP(+)).

