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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

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Related Experiment Video

Updated: Jun 3, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

Lectin affinity chromatography.

I West1, O Goldring

  • 1Department of Biological Sciences, North East Surrey College, Ewell, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
PubMed
Summary

Lectins are proteins with a specific affinity for carbohydrates. These purified proteins can be easily immobilized onto chromatography supports for various applications.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Affinity Chromatography

Background:

  • Lectins are proteins known for their carbohydrate-binding properties.
  • Their selective affinity makes them valuable tools in biological research.

Purpose of the Study:

  • To highlight the utility of lectins in biochemical applications.
  • To discuss the immobilization of lectins on chromatography supports.

Main Methods:

  • Purification of lectins.
  • Immobilization of lectins onto chromatography supports.

Main Results:

  • Purified lectins are commercially available.
  • Lectins can be successfully immobilized to various chromatography matrices.

More Related Videos

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Related Experiment Videos

Last Updated: Jun 3, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Conclusions:

  • The availability and immobilization of lectins offer versatile applications in affinity chromatography.
  • Lectins serve as effective tools for carbohydrate-based separation and analysis.