Lectin affinity chromatography

Related Experiment Video

Updated: Jun 3, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

I West1, O Goldring

  • 1Department of Biological Sciences, North East Surrey College, Ewell, Surrey, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
PubMed

Abstract:

Lectins are glycoproteins or proteins that have a selective affinity for a carbohydrate, or a group of carbohydrates. Many purified lectins are readily available and these maybe immobilized to a variety of chromatography supports.

More Related Videos

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Related Experiment Videos

Last Updated: Jun 3, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
20:23

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples

Published on: October 1, 2009

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

Articles linked to this work by shared authors, journal, and citation graph.

Lectin affinity chromatography.

Molecular biotechnology·1994

Reconstitution of neutrophil NADPH oxidase activity in the cell-free system by four components: p67-phox, p47-phox, p21rac1, and cytochrome b-245.

The Journal of biological chemistry·1992

Cytochrome b-245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytes.

The Biochemical journal·1992

Pesticide-induced illness. Public health aspects of diagnosis and treatment.

California medicine·1966

Preparation, Untargeted Metabolomic Analysis, and Short-Term Frozen Storage Evaluation of Leukocyte-Rich PRP (L-PRP) and Pure Platelet-Rich Plasma (P-PRP).

Methods in molecular biology (Clifton, N.J.)·2026

Targeted Screening of Traditional and Novel Stimulants in Dried Blood Spots Using LC-MS/MS.

Methods in molecular biology (Clifton, N.J.)·2026

Detection of Piperazines in Biological Samples Using Solid-Phase Extraction and Solid-Phase Microextraction by Gas Chromatography-Mass Spectrometry.

Methods in molecular biology (Clifton, N.J.)·2026

Determination of Tryptamines in Biological Specimens.

Methods in molecular biology (Clifton, N.J.)·2026

Liquid Chromatography Coupled to Mass Spectrometry for the Detection of Synthetic Opioids in Hair.

Methods in molecular biology (Clifton, N.J.)·2026

Determination of Novel Psychoactive Substances in Hair.

Methods in molecular biology (Clifton, N.J.)·2026

Structural modification, biological activities and applications of plant polysaccharides.

Carbohydrate research·2026

Photoredox/Cobalt-Catalyzed Stereoselective 2-Deoxyglycosylation of Glycals via MHAT-RPC with C/N/O-Nucleophiles.

Organic letters·2026

Convergent paired electrochemical dual C-H functionalization of pyrazolo[1,5-a]pyrimidines with cyanopyridines and ammonium halides.

Chemical communications (Cambridge, England)·2026

Guanidinium side-chain substitutions in the CPP-grafted p53-MDM2 inhibitor St3-R.

RSC medicinal chemistry·2026

Extracellular neuroligin-ICAM5 coupling drives dendritic growth via actin remodeling.

iScience·2026

USP Standards for Dietary Ingredients Derived from Mate and Guayusa Leaves: A Quality by Design Approach to Method Development and Validation for the Simultaneous Determination of Methylxanthines and Hydroxycinnamoylquinic Acids.

ACS omega·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us