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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
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In acid-base chemistry, the leveling effect refers to the limitation imposed by the solvent on the strength of acids and bases in solution. When a base stronger than the solvent's conjugate base is used, it deprotonates the solvent until the base is entirely consumed, making it ineffective against weaker acids. Conversely, an acid stronger than the solvent's conjugate acid protonates the solvent until the acid is depleted, rendering it ineffective against weaker bases. Essentially, the solvent...
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Hydrogen BondsHydrogen bonds are weak attractions between atoms that have formed other chemical bonds. One of these atoms is electronegative, like oxygen, and has a partial negative charge. The other is a hydrogen atom that has bonded with another electronegative atom and has a partial positive charge.Hydrogen Bonds Control the World!Because hydrogen has very weak electronegativity when it binds with a strongly electronegative atom, such as oxygen or nitrogen, electrons in the bond are...
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Few compounds act as strong acids. A far greater number of compounds behave as weak acids and only partially react with water, leaving a large majority of dissolved molecules in their original form and generating a relatively small amount of hydronium ions. Weak acids are commonly encountered in nature, being the substances partly responsible for the tangy taste of citrus fruits, the stinging sensation of insect bites, and the unpleasant smells associated with body odor. A familiar example of a...

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Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
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Exploiting weak affinities.

S Ohlson1

  • 1HyClone Laboratories, Inc., Logan, UT.

Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
PubMed
Summary
This summary is machine-generated.

Affinity chromatography is a separation technique utilizing specific molecular interactions. This method is crucial for isolating biochemical molecules like antigens and hormones.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Affinity chromatography (AC) is a powerful separation technique.
  • It relies on specific, reversible interactions between molecules.
  • Common examples include antibody-antigen and enzyme-inhibitor binding.

Purpose of the Study:

  • To elucidate the fundamental principles of affinity chromatography.
  • To highlight its applications in biochemical separations.
  • To provide a foundational understanding of AC for researchers.

Main Methods:

  • Utilizing specific binding pairs (e.g., antibody-antigen).
  • Employing a stationary phase with immobilized ligands.
  • Eluting target molecules based on binding affinity.

Main Results:

  • Demonstrated successful isolation of target biomolecules.
  • Showcased the specificity of molecular interactions in AC.
  • Validated the efficiency of AC for purification.

Conclusions:

  • Affinity chromatography is an effective method for purifying biomolecules.
  • The technique leverages highly specific molecular recognition.
  • AC is indispensable in various biochemical and biotechnological applications.