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Related Concept Videos

Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...
DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...

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Related Experiment Video

Updated: Jun 3, 2026

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography
11:18

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography

Published on: January 15, 2014

Making bacterial extracts suitable for chromatography.

R F Sherwood1

  • 1Centre for Applied Microbiology and Research, Porton Down, Salibury, Wiltshire, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
PubMed
Summary
This summary is machine-generated.

Bacterial extract preparation focuses on clarification and concentration for chromatography. Methods impact extract properties, with advanced techniques enabling larger-scale processing.

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Last Updated: Jun 3, 2026

Isolation and Preparation of Bacterial Cell Walls for Compositional Analysis by Ultra Performance Liquid Chromatography
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Published on: January 15, 2014

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Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Chromatography

Background:

  • Bacterial extracts are primarily water, requiring preparation for downstream applications.
  • Cell disruption methods can influence extract properties, affecting clarification efficiency.

Purpose of the Study:

  • To outline essential clarification and concentration techniques for bacterial extracts prior to chromatography.
  • To discuss methods applicable to pilot and large-scale processing.

Main Methods:

  • Clarification techniques include centrifugation, two-phase separation, and filtration.
  • Concentration methods involve filtration, precipitation, and batch binding to ion-exchange matrices.
  • Consideration of physical, chemical, and mechanical methods for extract preparation.

Main Results:

  • Various physical and chemical methods are effective for clarifying and concentrating bacterial extracts.
  • Protein precipitation and batch binding serve as primary purification steps during concentration.
  • Fast-flow chromatographic matrices allow direct application of larger process volumes.

Conclusions:

  • Effective clarification and concentration are crucial for preparing bacterial extracts for chromatography.
  • The choice of preparation method impacts extract properties and subsequent processing.
  • Advancements in chromatography enable more efficient large-scale processing of bacterial extracts.