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Related Concept Videos

Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Updated: Jun 3, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

RIP: an mRNA localization technique.

Sabarinath Jayaseelan1, Francis Doyle, Salvatore Currenti

  • 1Department of Nanobioscience, University at Albany, Albany, NY, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 25, 2011
PubMed
Summary
This summary is machine-generated.

Understanding post-transcriptional gene expression requires studying RNA-protein interactions. Ribonomic profiling using RNA-binding protein immunoprecipitation (RIP) isolates mRNA to reveal gene regulation and localization.

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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
10:24

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

Published on: December 17, 2012

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Post-transcriptional gene expression involves complex RNA-protein interactions.
  • Messenger RNA (mRNA) profiling is crucial for understanding cellular biomolecular machinery.
  • RNA-binding proteins (RBPs) play key roles in regulating mRNA fate.

Purpose of the Study:

  • To detail the methodology for mRNA isolation using RBP immunoprecipitation (RIP).
  • To provide a comprehensive guide for ribonomic profiling techniques.
  • To facilitate the study of post-transcriptional gene regulation and mRNA localization.

Main Methods:

  • Utilizing RBP immunoprecipitation (RIP) as the primary method for mRNA isolation.
  • Employing specific antibodies to target and capture RBPs and their associated mRNA.
  • Describing the RIP-Chip or ribonomic profiling technique for in vivo analysis.

Main Results:

  • Successful isolation of mRNA associated with specific RBPs.
  • Demonstration of RIP as a versatile technique for studying mRNA profiles.
  • Provides a detailed protocol for researchers.

Conclusions:

  • RBP immunoprecipitation is an effective method for mRNA isolation.
  • Ribonomic profiling offers insights into post-transcriptional gene regulation.
  • This methodology aids in correlating RNA-protein interactions with cellular functions.