[Effect of thermo-chemo therapy on human small cell lung cancer H446 cell apoptosis]
Lin Wang1, Xinkui Liu, Shan Gao
1Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. darwomen@yahoo.com.cn
Objective:
To study the synergistic effect of thermo-chemo therapy on lung cancer cells and its possible mechanisms.
Methods:
Refer the normal clinic dose, and use the untreated H446 cells as the control group. H446 cells were treated by different thermo-chemo therapy strategies: 43 degrees C + Paclitaxel (120 microg/L) (thermo-chemotherapy group), 43 degrees C + Paclitaxel (120 microg/L) + NAC (30 micromol/L, specific reactive oxygen species (ROS) inhibitor) (NAC group), and Paclitaxel (120 microg/L) alone (only chemotherapy group). Cell apoptosis was analyzed by FCM and fluorescence was used to measure ROS inside the cells. The expressions of Caspase-3 was determined by Westerm Blotting. Use SPSS 13.0 to perform the statistical analysis on the data.
Results:
The rate of cell apoptosis in the thermo-chemo therapy group was significantly higher than those in the other groups (P < 0.05). The ROS in the thermo-chemo therapy group increased (P < 0.05), but NAC inhibited its expression. The expression of Caspase-3 in the thermo-chemo therapy group increased significantly (P < 0.05), but can be inhibited by NAC (P < 0.05).
Conclusion:
Thermo-chemo therapy can significantly seduce the apotosis of H446 cells, probably through seducing ROS forming, realised by the caspase channel.
