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Updated: Jun 3, 2026
Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)
Published on: March 11, 2020
[Effects of different factors on transformed hairy root in Gynostemm apentaphyllum]
Feng-Fa He1, Tian Tian, Gui-Xue Wang
1College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China. hefengfa@126.com
Objective:
To induce hairy roots of Gynostemm apentaphyllum by Agrobacterium rhizogenes strains.
Methods:
Hairy roots were induced by the co-culture method of explants and Agrobacterium rhizogenes strains. Effects of different Agrobacterium rhizogenes strains, explants, pre(co)-culture time, Bacterial concentration, infecting time, As concentration and antibiotic medium on the transformation frequency were studied.
Results:
The highest induction frequency was obtained form leaf 2 days co-cultivation, which were induced by Agrobacterium rhizogenes OD 600 0. 8 for 10 min, 100 micromol/L As and MS + 300 mg/L Cab.
Conclusion:
Hairy roots were induced by co-cultivation and the optimum induced condition were determined.
