[Cloning and sequence analysis on functional genes of flavonoid biosynthesis in Citrus grandis 'tomenxosa']

Cloned several important functional genes from Citrus grandis 'tomentosa', which was the genuine medicinal materials from Guangdong province.

CTAB-LiCl treatment was used to extract RNA from Citrus grandis 'tomentosa', the technology of RT-PCR was used to clone the genes.

Three genes were cloned from Citrus grandis 'tomentosa', which were Chalcone Synthase (CHS) gene, Flavanone 3-hydroxylase (F3H) gene and Chalcone isomerase (CHI) gene. The open reading frame (ORF) of CHS gene was consisted of 1173 bp, and encoded 391 amino acid residues, that of F3H gene was consisted of 1086 bp, and encoded 362 amino acid residues, that of CHI gene was consisted of 666 bp, and encoded 222 amino acid residues. The accession number of three genes were GQ892059, GU323284 and GU323285 respectively.

CTAB-LiCl treatment could extract high quality RNA from Citrus grandis 'tomentosa', which could be used to follow-up work. The three functional genes which were cloned from Citrus grandis 'tomentosa' had ORFs.