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Updated: Jun 3, 2026

Processing Embryo, Eggshell, and Fungal Culture for Scanning Electron Microscopy
Published on: August 16, 2019
Embryonic development of Anodontites trapesialis (Lamarck, 1819) (Bivalvia: Mycetopodidae)
A T Silva-Souza1, P Guardia-Felipi, N R Arrebola
1Laboratório de Ecologia de Parasitos de Organismos Aquáticos, Departamento de Biologia Animal e Vegetal, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, PR, Brazil. ateresa@uel.br
Abstract:
The phases of embryonic development of Anodontites trapesialis lasidia are described for the first time. Adult specimens were obtained from two fish farms located in Londrina, Paraná, Brazil. The internal demibranchs of 120 individuals were studied using a routine histological technique; 70 of these carried eggs and/or larvae in the marsupium and were utilized for the description of the phases of embryonic development. The demibranchs of five specimens were evaluated by scanning electron microscopy to detail the morphology of the larvae. Five phases of development were established: phase I, corresponding to the initial stage of cleavage with the formation of apical cells; phase II, including the stages of the morula and blastula; phase III, where the gastrula forms; phase IV, where the larva formed is still inside the egg envelope; and phase V, where the lasidium can still be identified immediately after eclosion.
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