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Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Published on: October 20, 2011
Changes in the subgingival biofilm composition after coronally positioned flap
Jadson Almeida Lima1, Vanessa Renata Santos, Magda Feres
1Department of Periodontics, Dental Research Division, Guarulhos University, Guarulhos, SP, Brazil.
Journal of Applied Oral Science : Revista FOB
|March 26, 2011
Summary
Coronally positioned flap (CPF) surgery improved gum health and reduced harmful bacteria in subgingival biofilms. This periodontal treatment positively altered the oral microbiome composition six months post-surgery.
Area of Science:
- Periodontology
- Microbiology
- Regenerative Dentistry
Background:
- Gingival recession affects oral health and aesthetics.
- Subgingival biofilm composition is crucial in periodontal disease progression.
Purpose of the Study:
- To evaluate the impact of coronally positioned flap (CPF) surgery on subgingival biofilm composition.
- To assess changes in periodontal pathogens following CPF treatment.
Main Methods:
- Twenty-two subjects with gingival recession underwent CPF surgery.
- Clinical parameters and subgingival biofilm samples were analyzed pre- and post-surgery (6 months).
- Checkerboard DNA-DNA hybridization identified 40 bacterial species in biofilms.
Main Results:
- Significant improvements in recession height, clinical attachment level, and bleeding on probing were observed (p<0.05).
- Proportions of 10 periodontal pathogens decreased significantly.
- Red and orange complex bacterial proportions were reduced post-CPF.
Conclusions:
- Coronally positioned flap surgery positively influences subgingival microbiota composition.
- CPF treatment leads to a reduction in key periodontal pathogens.
- Beneficial shifts in the oral microbiome are evident six months after CPF surgery.
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