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Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells
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S-phase-coupled apoptosis in tumor suppression

Yong-Jig Cho1, Peng Liang

  • 1Department of Cancer Biology, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA. y.cho@vanderbilt.edu

Cellular and Molecular Life Sciences : CMLS
|March 26, 2011
PubMed

Insights

Cell cycle checkpoints ensure genomic integrity by controlling DNA replication. This review highlights how S-phase checkpoint control, involving factors like Killin, guides cell fate decisions to prevent replication errors.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DNA replication ensures accurate genetic information transfer between cell generations.
  • This process is tightly regulated by positive and negative factors.
  • Replication errors or DNA damage trigger checkpoint pathways.

Purpose of the Study:

  • To review the critical role of S-phase checkpoint control in mammalian cells.
  • To emphasize the involvement of Killin in safeguarding genome integrity.
  • To explore the cell's life and death decisions during replication stress.

Main Methods:

  • Literature review focusing on DNA replication and checkpoint control.
  • Analysis of evidence for S-phase checkpoint mechanisms.
  • Examination of the role of specific proteins like Killin, ATM/ATR, CHK kinases, and p53.

Main Results:

  • S-phase checkpoint activation is induced by replication stress or DNA damage.
  • Checkpoint pathways, including ATM/ATR, CHK kinases, and p53, mediate damage control.
  • Killin is a recently identified factor influencing these decisions.

Conclusions:

  • S-phase checkpoint control is essential for maintaining mammalian genome integrity.
  • Cells utilize these checkpoints to make critical life-or-death decisions.
  • Understanding these pathways, including Killin's role, is vital for preventing genomic instability.

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