Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Matrix Vesicles Versus Exosomes: A Comparative Study on Their Ability to Promote Growth Plate Mineralization and Ectopic Calcification.

Frontiers in bioscience (Landmark edition)·2026
Same author

Biomolecular Corona Remodelling of <i>Aspergillus fumigatus</i> Extracellular Vesicles Shapes Immune Recognition and Macrophage Activation Programs.

Journal of extracellular biology·2026
Same author

Digitally Coded, Screen-Printed Flexible Metasurfaces for Tunable Electromagnetic Responses.

ACS applied materials & interfaces·2026
Same author

Inhibition of sphingomyelinase blocks the recruitment of alkaline phosphatase to mineralization-competent matrix vesicles.

Bone·2026
Same author

Bioinspired Engineering of <i>Bombyx mori</i> Silk Fibroin: Solution Blow-Spun Nanofibers Hybridized with (3-Aminopropyl)triethoxysilane.

ACS applied bio materials·2026
Same author

Multipolar decomposition of magnetic circular dichroism in arbitrarily shaped magneto-dielectric scatterers.

Optics express·2026

Related Experiment Video

Updated: Jun 3, 2026

Near Infrared Optical Projection Tomography for Assessments of &beta;-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Using multidimensional projection techniques for reaching a high distinguishing ability in biosensing.

Fernando V Paulovich1, Rafael M Maki, Maria C F de Oliveira

  • 1Instituto de Ciências Matemáticas e de Computação, USP, CP, São Carlos, São Paulo, Brazil.

Analytical and Bioanalytical Chemistry
|March 26, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces Projection Explorer (PEx), an information visualization system that enhances biosensor performance. PEx successfully distinguishes between diseases like Chagas and Leishmaniasis using impedance spectroscopy data, outperforming traditional methods.

More Related Videos

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Related Experiment Videos

Last Updated: Jun 3, 2026

Near Infrared Optical Projection Tomography for Assessments of &beta;-cell Mass Distribution in Diabetes Research
15:18

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
11:54

Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles

Published on: March 13, 2017

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

  • Biosensing and molecular engineering
  • Information visualization for data analysis
  • Nanostructured materials in sensor development

Background:

  • Advanced molecular engineering enables precise molecular recognition in biosensing for clinical and environmental applications.
  • Large datasets from biosensors necessitate sophisticated data processing to optimize performance.
  • Nanostructured films with immobilized antigens are key components in high-performance biosensors.

Purpose of the Study:

  • To demonstrate the efficacy of the Projection Explorer (PEx) information visualization system for optimizing biosensor performance.
  • To apply PEx to impedance spectroscopy data from nanostructured film biosensors for specific analyte detection.
  • To differentiate between complex biological samples using advanced data visualization techniques.

Main Methods:

  • Utilized impedance spectroscopy to collect data from an array of biosensors with immobilized antigens.
  • Employed the Projection Explorer (PEx) system for data visualization and analysis, including projection and normalization methods.
  • Applied Sammon's mapping with standardization for data normalization to achieve optimal distinction.

Main Results:

  • PEx achieved high performance in biosensing, enabling specific recognition of antibodies (analytes).
  • Successfully distinguished between positive Chagas disease and Leishmaniasis samples, a feat not possible with conventional statistical methods.
  • Identified Sammon's mapping with standardization as the optimal method, detecting antibodies at concentrations as low as 10(-7) mg/mL.

Conclusions:

  • The PEx system and its associated impedance data analysis procedures offer a generic and extensible approach for optimizing various sensor and biosensor types.
  • Information visualization significantly enhances the analytical capabilities of biosensors, particularly for complex datasets.
  • This methodology holds promise for improved clinical diagnosis and environmental monitoring through advanced biosensing data interpretation.