Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Chemical Ionization (CI) Mass Spectrometry

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Cross-cultural differences in social and self-referential memory are magnified with age.

Neuropsychology, development, and cognition. Section B, Aging, neuropsychology and cognition·2026

Same author

Dual SYK-HDAC Inhibitor Elicits Striking Efficacy against Acute Myeloid Leukemia: Rational Design, Synthesis, and Biological Evaluation.

Journal of medicinal chemistry·2026

Same author

Refining cognitive dispersion metrics for brain-behavior prediction in aging and mild cognitive impairment.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026

Same author

Baicalin suppresses colorectal cancer proliferation and induces M1 polarization of tumor-associated macrophages by promoting proteasomal degradation of HK2.

Frontiers in immunology·2026

Same author

The efficiency of 0.01% topical atropine on the myopia prevention in low refractive error population with different refractive errors: a retrospective cohort study.

BMC ophthalmology·2026

Same author

Adverse Reactions and Vaccination Preferences Following COVID-19 and Influenza Vaccination Among Healthcare Workers: A Cross-Sectional Survey.

The Kaohsiung journal of medical sciences·2026

Search research articles

Related Experiment Video

Updated: Jun 3, 2026

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

Ionic liquid-assisted electrospray ionization of polysaccharides.

Yu-Ling Chang¹, Yuan-Chun Lee, Wen-Bin Yang

¹Genomics Research Center, Academia Sinica, Nankang, Taipei, Taiwan.

Journal of Mass Spectrometry : JMS

|March 26, 2011

Summary

Ionic liquid-assisted electrospray ionization (ILA-ESI) mass spectrometry enhances polysaccharide detection sensitivity. This novel method simplifies mass spectra, improving the analysis of large neutral carbohydrate compounds.

More Related Videos

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Related Experiment Videos

Last Updated: Jun 3, 2026

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Published on: February 19, 2020

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

Area of Science:

Analytical Chemistry
Mass Spectrometry
Carbohydrate Chemistry

Background:

Electrospray ionization (ESI) mass spectrometry is crucial for analyzing biomolecules.
Analyzing large neutral polysaccharides presents challenges in detection sensitivity and spectral complexity.
Existing methods often yield complex mass spectra, hindering accurate identification.

Purpose of the Study:

To significantly improve the detection sensitivity of polysaccharides using ESI.
To simplify mass spectra of polysaccharides for enhanced analysis.
To investigate the effect of ionic liquids on ESI mass spectra of polysaccharides.

Main Methods:

Addition of various ionic liquid compounds to polysaccharide samples.
Analysis using electrospray ionization (ESI) mass spectrometry.
Observation and comparison of mass spectra with and without ionic liquid additives.

Main Results:

Achieved significant improvement in detection sensitivity for polysaccharides.
Observed simplified mass spectra, resembling matrix-assisted laser desorption/ionization (MALDI).
Noted narrow charge number distribution in mass spectra.
Detected polysaccharide ions with attached ionic liquid anions or cations.
Observed minimal protonated/deprotonated or alkali-attached polysaccharide ions.

Conclusions:

Ionic liquid-assisted ESI (ILA-ESI) mass spectrometry substantially enhances the detection sensitivity of large neutral polysaccharides.
ILA-ESI simplifies mass spectra, aiding in the characterization of complex carbohydrate structures.
This technique offers a promising alternative for sensitive polysaccharide analysis.