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Updated: Jun 3, 2026

Preparation of Chloroplast Sub-compartments from Arabidopsis for the Analysis of Protein Localization by Immunoblotting or Proteomics
Published on: October 19, 2018
Multiple FtsZ2 isoforms involved in chloroplast division and biogenesis are developmentally associated with thylakoid
Mohamed Karamoko1, El-Sayed El-Kafafi, Paul Mandaron
1Laboratoire de Physiologie Cellulaire Végétale, CNRS-(UMR5168)/INRA-(UMR1200)/UJF-Grenoble 1/CEA-iRTSV, Institut de Recherches en Technologies et Sciences pour le Vivant, CEA-Grenoble, 17 Rue des Martyrs, F-38054 Grenoble, France.
Abstract:
Seed plants and algae have two distinct FtsZ protein families, FtsZ1 and FtsZ2, involved in plastid division. Distinctively, seed plants and mosses contain two FtsZ2 family members (FtsZ2-1 and FtsZ2-2) thus raising the question of the role of these FtsZ2 paralogs in plants. We show that both FtsZ2 paralogs, in addition to being present in the stroma, are associated with the thylakoid membranes and that association is developmentally regulated. We also show that several FtsZ2-1 isoforms are present with distinct intra-plastidial localization. Mutant analyses show that FtsZ2-1 is essential for chloroplast division and that FtsZ2-2 plays a specific role in chloroplast morphology and internal organisation in addition to participating in chloroplast partition.
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