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Decrease in N-ethylmaleimide-sensitive ATPase activity in collecting duct by metabolic alkalosis.

L C Garg1, N Narang

  • 1Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville 32610.

Canadian Journal of Physiology and Pharmacology
|August 1, 1990
PubMed
Summary
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Metabolic alkalosis reduces N-ethylmaleimide (NEM)-sensitive proton pump (H-ATPase) activity in rat kidney collecting ducts. This suggests acid-base status influences proton secretion in the distal nephron.

Area of Science:

  • Nephrology
  • Renal Physiology
  • Biochemistry

Background:

  • Systemic acid-base balance impacts renal acidification processes.
  • Proton (H+) secretion in the collecting duct is electrogenic.
  • N-ethylmaleimide (NEM)-sensitive H+-ATPase is implicated in renal H+ secretion.

Purpose of the Study:

  • To investigate the effect of metabolic alkalosis on NEM-sensitive ATPase activity.
  • To assess activity in microdissected segments of the rat distal nephron.

Main Methods:

  • Metabolic alkalosis induced in rats via NaHCO3 administration for 7 days.
  • Measurement of NEM-sensitive ATPase activity in microdissected cortical and medullary collecting duct segments.
  • Comparison of enzyme activity between alkalotic and control rats.

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Main Results:

  • Significantly lower NEM-sensitive ATPase activity observed in cortical, outer medullary, and inner medullary collecting ducts of alkalotic rats.
  • No significant difference in enzyme activity in other examined nephron segments.
  • Plasma total CO2 levels confirmed metabolic alkalosis in the experimental group.

Conclusions:

  • NEM-sensitive H+-ATPase activity in all segments of the collecting duct is modulated by the animal's acid-base status.
  • Findings suggest a regulatory role for acid-base balance in renal proton secretion mechanisms.