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Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
Published on: January 9, 2026
Mesoporous carbon microparticles as a novel fluorescent sensing platform for thrombin detection
Yingwei Zhang1, Sen Liu, Xuping Sun
1State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 5625 Renmin Street, Changchun 130022, Jilin, China.
Biosensors & Bioelectronics
|March 29, 2011
Summary
This study introduces novel MC microparticles for detecting thrombin (TB) using fluorescence. The method offers a sensitive and selective platform for TB detection, even in human blood serum.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Thrombin (TB) is a key biomarker in hemostasis and thrombosis.
- Developing sensitive and selective detection methods for TB is crucial for clinical diagnostics.
- Fluorescent sensing platforms offer high sensitivity for biomolecule detection.
Purpose of the Study:
- To develop a novel fluorescent sensing platform for thrombin detection using MC microparticles (MCMPs).
- To investigate the mechanism of fluorescence quenching and recovery based on thrombin binding.
- To evaluate the performance of the developed sensor in complex biological matrices like human blood serum.
Main Methods:
- MC microparticles (MCMPs) were synthesized using a nanocasting method with mesoporous silica (MS) nanoparticles as templates.
- Dye-labeled thrombin-binding aptamer (TA) was adsorbed onto MCMPs via π-π stacking.
- Fluorescence quenching was observed due to the proximity of the dye to the MCMP surface.
- Thrombin detection was achieved by monitoring the recovery of fluorescence upon formation of a thrombin-aptamer complex.
Main Results:
- The MCMP-based platform demonstrated a low detection limit for thrombin down to 0.25 nM.
- The sensing system exhibited excellent selectivity for thrombin over other proteins.
- The fluorescence quenching mechanism was effectively utilized for thrombin quantification.
- Successful application of the sensor in detecting thrombin in human blood serum was demonstrated.
Conclusions:
- MC microparticles provide a novel and effective platform for fluorescent thrombin sensing.
- The developed method offers high sensitivity and selectivity for thrombin detection.
- This approach has potential applications in clinical diagnostics and biological sample analysis.
