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Updated: Jun 3, 2026
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Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
Published on: April 11, 2013
Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root
Yun Zeng Zhang1, Wen Feng Chen1, Mao Li1
1State Key Laboratory of Agrobiotechnology and Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing 100193, PR China.
Summary
A novel bacterium, Bacillus endoradicis sp. nov., was discovered in soybean roots. This gram-positive, aerobic bacterium represents a new species within the Bacillus genus, distinct from its closest relatives.
Area of Science:
- Microbiology
- Plant Science
- Bacteriology
Background:
- Soybean (Glycine max L.) roots harbor diverse microbial communities.
- Understanding root-associated bacteria is crucial for plant health and agricultural sustainability.
Purpose of the Study:
- To isolate and characterize a novel bacterial species from soybean root tissues.
- To determine the phylogenetic and phenotypic distinctiveness of the novel isolate.
Main Methods:
- Isolation of bacteria from soybean root inner tissues.
- Phylogenetic analysis using 16S rRNA gene sequencing.
- Phenotypic characterization including growth conditions, biochemical tests, and cellular fatty acid analysis.
- DNA-DNA hybridization for genetic relatedness assessment.
Main Results:
- A novel gram-positive, aerobic, motile rod, strain CCBAU 05776(T), was isolated.
- Phylogenetic analysis showed <97% 16S rRNA gene similarity to Bacillus muralis and Bacillus simplex.
- Phenotypic tests revealed differences in casein and starch hydrolysis, and MacConkey agar growth.
- DNA-DNA relatedness values were low (<43%) with related species.
- The DNA G+C content was 40.8 mol%.
Conclusions:
- Strain CCBAU 05776(T) represents a novel species within the genus Bacillus.
- The proposed name for this new species is Bacillus endoradicis sp. nov.
- The type strain is CCBAU 05776(T).
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