Related Experiment Video
Updated: Jun 3, 2026
07:43
An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
Published on: August 23, 2016
New model systems provide insights into Myc-induced transformation.
A R Wasylishen1, A Stojanova, S Oliveri
1Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada.
Oncogene
|March 29, 2011
Summary
Myc
Area of Science:
- Oncology
- Molecular Biology
- Cell Biology
Background:
- Myc is a potent oncoprotein driving cellular transformation and tumorigenesis.
- Understanding Myc's mechanisms is crucial for developing targeted cancer therapies.
- Rodent fibroblast models have historically informed Myc function studies.
Purpose of the Study:
- To develop and characterize novel human cell line models for studying Myc-dependent transformation.
- To evaluate the oncogenic potential of various Myc family proteins and mutants in these new models.
- To investigate the role of cellular context in Myc-mediated tumorigenesis.
Main Methods:
- Characterization of three novel human cell lines: MCF10A, SH-EP Tet21/N-Myc, and LF1/TERT/LT/ST.
- Assessment of Myc family proteins (c-Myc, L-Myc), MycS isoform, and N-terminal mutants (ΔMBII, W135E, T58A).
- Evaluation of anchorage-independent growth as a measure of transformation potential.
Main Results:
- Successfully established and characterized three new human cell-based models for Myc research.
- Demonstrated varying abilities of different Myc proteins and mutants to induce anchorage-independent growth.
- Identified that Myc's role in tumorigenesis may not be exclusively dependent on the MBII domain.
Conclusions:
- The developed human cell lines offer valuable tools for studying Myc's role in cancer.
- Cellular context significantly influences Myc's oncogenic activity.
- The findings challenge existing paradigms regarding the essentiality of the MBII domain for Myc-driven tumorigenesis.
More Related Videos
Related Concept Videos
02:43Mouse Models of Cancer Study
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
01:06Induced Pluripotent Stem Cells
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Somatic cells are...
02:23Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...