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Related Experiment Videos

Elastase-induced experimental aneurysms in rats.

S Anidjar1, J L Salzmann, D Gentric

  • 1INSERM U36-17, Paris, France.

Circulation
|September 1, 1990
PubMed
Summary

Elastase infusion causes aortic aneurysms in rats by destroying elastic tissue. Activated macrophages may secrete elastase, contributing to aneurysm development and progression.

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Area of Science:

  • Vascular Biology
  • Biochemistry
  • Pathology

Background:

  • Aortic aneurysms involve the destruction of elastic tissue in the aortic wall.
  • The role of specific enzymes and cellular mediators in aneurysm pathogenesis requires further elucidation.

Purpose of the Study:

  • To establish an in vivo rat model of aortic aneurysm using pancreatic elastase.
  • To investigate the role of elastase and activated macrophages in aortic aneurysm formation and elastic tissue degradation.

Main Methods:

  • Perfusion of isolated rat abdominal aortas with pancreatic elastase or thioglycollate-activated macrophages.
  • Dose-response assessment of elastase concentration on aneurysm formation.
  • Evaluation of elastic tissue integrity and elastolytic activity in aortic tissue.

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Main Results:

  • Elastase perfusion induced dose-dependent aneurysmal dilation and elastic tissue loss.
  • Activated macrophages in the aortic media caused aortitis and elastic tissue destruction without true aneurysm.
  • Combined elastase and plasmin perfusion significantly increased elastolytic activity and aggravated aneurysmal lesions.

Conclusions:

  • Elastase presence in the aortic media is a key factor in aneurysm formation.
  • Activated macrophages may contribute to elastase secretion and subsequent elastic tissue destruction.
  • Plasmin may potentiate elastase activity, exacerbating aortic aneurysm development.