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Pigtailed macaque model refinement: topical microbicide safety in the presence of coitus
Yvonne T Cosgrove Sweeney1, Ray O Angeles, Peter K Cummings
1Department of Obstetrics & Gynecology, University of Washington, Seattle, WA, USA.
Background:
Inclusion of sexual activity in the macaque model for topical microbicide safety evaluation would more closely mimic human use of topical microbicides and provide a more rigorous safety assessment.
Methods:
Initially, male-female partners were monitored in cohousing arrangements to determine whether macaques would copulate ad libitum. The logistics of performing vaginal examinations before and after coital visits were analyzed and optimized. Findings from cervicovaginal examinations conducted before and after sexual activity were compared.
Results:
Coital activity was reliably observed in the majority of cohousing sessions, representing all phases of the menstrual cycle. Female macaques were trained to be restrained while fully alert for pre-coital vaginal sampling. Post-coital examinations occur under general sedation. Post-coital examinations reveal alterations to tissues, microbiology, and pH compared with pre-coital visits.
Conclusions:
This work clearly demonstrates that it is feasible to incorporate sexual activity in the macaque model for topical microbicide safety assessment.
