Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measuring effect of mutations & conditions on microbial respiratory rates.

Journal of microbiological methods·2023
Same author

Investigating cell autonomy in microorganisms.

Current genetics·2022
Same author

How Boundaries Form: Linked Nonautonomous Feedback Loops Regulate Pattern Formation in Yeast Colonies.

Genetics·2019
Same author

Shrinking Daughters: Rlm1-Dependent G<sub>1</sub>/S Checkpoint Maintains <i>Saccharomyces cerevisiae</i> Daughter Cell Size and Viability.

Genetics·2017
Same author

Similar environments but diverse fates: Responses of budding yeast to nutrient deprivation.

Microbial cell (Graz, Austria)·2016
Same author

Phenotypic plasticity within yeast colonies: differential partitioning of cell fates.

Current genetics·2016
Same journal

A Video Protocol of a Randomized Controlled Clinical Trial - Electrochemotherapy of Cutaneous Metastases with Reduced Dose Bleomycin (BLESS Trial).

Journal of visualized experiments : JoVE·2026
Same journal

A Standardized Ex Vivo Porcine Oromucosal Model for Evaluating Peptide Fluxes.

Journal of visualized experiments : JoVE·2026
Same journal

Lightweight English Text Classification with Deep Learning Based on Complex System Theory.

Journal of visualized experiments : JoVE·2026
Same journal

Integrating Artificial Intelligence-Assisted Translation Support into English Courses: Effects on Translation Accuracy, Perceived Stress, and Anxiety.

Journal of visualized experiments : JoVE·2026
Same journal

A Toxin-Based Counter-Selection System for Markerless Gene Deletion and High-Density Tn5 Transposon Mutagenesis in Pectobacterium brasiliense.

Journal of visualized experiments : JoVE·2026
Same journal

Seamless Multimodal Human-Robot Communication: Integration Techniques in Human-Computer Interaction.

Journal of visualized experiments : JoVE·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

Yeast Colony Embedding Method
09:04

Yeast Colony Embedding Method

Published on: March 22, 2011

Yeast colony embedding method.

Sarah Piccirillo1, Saul M Honigberg

  • 1School of Biological Sciences, University of Missouri - Kansas City, USA.

Journal of Visualized Experiments : Jove
|March 30, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to embed and section yeast colonies, revealing internal cell type patterns. This technique allows detailed visualization of fungal colony structures for better understanding of microbial development.

More Related Videos

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
08:52

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Published on: March 23, 2018

Cryosectioning Yeast Communities for Examining Fluorescence Patterns
07:03

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Published on: December 26, 2012

Related Experiment Videos

Last Updated: Jun 3, 2026

Yeast Colony Embedding Method
09:04

Yeast Colony Embedding Method

Published on: March 22, 2011

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis
08:52

Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Published on: March 23, 2018

Cryosectioning Yeast Communities for Examining Fluorescence Patterns
07:03

Cryosectioning Yeast Communities for Examining Fluorescence Patterns

Published on: December 26, 2012

Area of Science:

  • Microbiology
  • Developmental Biology
  • Microscopy

Background:

  • Cell type patterning is crucial in metazoan development and microbial communities.
  • Fungal colonies, like yeast, exhibit complex cell type patterns (e.g., sporulated, pseudohyphal cells).
  • Investigating these patterns is challenging due to weak cell adhesion and lack of extensive extracellular matrix in fungi.

Purpose of the Study:

  • To develop a novel method for embedding and sectioning yeast colonies.
  • To enable detailed visualization of internal cell type patterns within fungal colonies.
  • To facilitate the study of molecular mechanisms underlying microbial colony development.

Main Methods:

  • Embedding small yeast colonies (1-2 mm diameter) in agar.
  • Infiltrating embedded colonies with Spurr's medium for stabilization.
  • Sectioning embedded colonies to produce thick (0.5 μm) and thin (0.1 μm) sections.
  • Utilizing light microscopy and transmission electron microscopy (TEM) for visualization.

Main Results:

  • Successfully visualized internal cell type patterns in yeast colonies.
  • Distinguished between different cell types (asci, pseudohyphal, ovoid yeast cells) using light microscopy.
  • Revealed interior cellular structures using TEM.
  • Enabled visualization of the region where the colony invades the underlying agar.

Conclusions:

  • The developed embedding and sectioning method effectively reveals internal structures of yeast colonies.
  • This technique is valuable for studying microbial development and cell type patterning.
  • The method supports both light and electron microscopy, offering versatile applications in mycology and microbiology.