Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

17.0K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.0K
Cells Coordinate Growth and Proliferation02:36

Cells Coordinate Growth and Proliferation

5.0K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
5.0K
Bacterial Signaling01:30

Bacterial Signaling

39.9K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
39.9K
Global Regulatory Systems01:28

Global Regulatory Systems

535
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
535
Cell Signaling Feedback Loops01:07

Cell Signaling Feedback Loops

7.2K
Positive and negative feedback loops are crucial for regulating biological signaling systems. These feedback loops are processes that connect output signals to their inputs.
Negative feedback loops
Most signaling systems have negative feedback loops that can perform different functions such as output limiter, and adaptation.
Output limiter
Upon receiving an input signal, the cellular response rapidly increases until a threshold is reached. Beyond this threshold, a negative feedback loop...
7.2K
Operon Model01:23

Operon Model

1.0K
The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
1.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative Structural Modeling of Intrinsically Disordered Regions in a Human HDAC2 Chromatin Remodeling Complex.

bioRxiv : the preprint server for biology·2026
Same author

Proteomic analysis of FACS-enriched whole nematocysts from the colonial hydrozoan <i>Hydractinia symbiolongicarpus</i>.

Toxicon: X·2026
Same author

Inhibiting sirtuin-dependent DNA repair and oxidative stress responses impairs DIPG cell survival.

Research square·2025
Same author

Somatic CRISPR tumorigenesis and multiomic analysis reveal a pentose phosphate pathway disruption vulnerability in MPNSTs.

Science advances·2025
Same author

Live Cell Imaging of Bone Cell and Organ Cultures.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Additive Effects of Cu-ATSM and Radiation on Survival of Diffuse Intrinsic Pontine Glioma Cells.

Radiation research·2024
Same journal

Inherited long telomeres induce a genome-wide transcriptional response in budding yeast.

Genetics·2026
Same journal

Adaptive Dynamics of Quantitative Traits in a Steadily Changing Environment.

Genetics·2026
Same journal

Functional Landscape of Zebrafish Gonadotropins and Receptors: A Comprehensive Genetic Analysis.

Genetics·2026
Same journal

Synergistic actions of Nup43 and Myosin VI drive actin cone assembly during Drosophila spermiogenesis.

Genetics·2026
Same journal

Identification of two Cryptococcus neoformans heme transporters involved in Fhb1-mediated nitrosative stress protection in a fission yeast model.

Genetics·2026
Same journal

Analysis of a hypomorphic mei-P26 mutation reveals coordination between developmental programming of germ cells and meiotic chromosome dynamics.

Genetics·2026
See all related articles

Related Experiment Video

Updated: Jan 5, 2026

Yeast Colony Embedding Method
09:04

Yeast Colony Embedding Method

Published on: March 22, 2011

11.8K

How Boundaries Form: Linked Nonautonomous Feedback Loops Regulate Pattern Formation in Yeast Colonies.

Sarah Piccirillo1, Abbigail H McCune1, Samuel R Dedert1

  • 1Division of Cell Biology and Biophysics, School of Biological and Chemical Sciences, University of Missouri-Kansas City, Missouri 64110.

Genetics
|October 18, 2019
PubMed
Summary
This summary is machine-generated.

Two linked signaling pathways control yeast colony development. A cell-nonautonomous feedback loop activates cell differentiation, ensuring proper pattern formation in budding yeast colonies.

Keywords:
Ime1Rim101Rlm1Slt2cell autonomycell–cell signaling

More Related Videos

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

419
Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

2.6K

Related Experiment Videos

Last Updated: Jan 5, 2026

Yeast Colony Embedding Method
09:04

Yeast Colony Embedding Method

Published on: March 22, 2011

11.8K
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

419
Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

2.6K

Area of Science:

  • Cell biology
  • Developmental biology
  • Microbiology

Background:

  • Budding yeast colonies exhibit distinct layers of meiotic and feeder cells.
  • Colony differentiation relies on the Rlm1/cell-wall integrity and Rim101/alkaline-response pathways.

Purpose of the Study:

  • Investigate the interplay between Rlm1 and Rim101 signaling in yeast colony patterning.
  • Elucidate the cell-autonomy and expression patterns of these pathways.

Main Methods:

  • Analysis of gene expression patterns.
  • Determination of cell-autonomy relationships.
  • Investigating signaling pathway interactions.

Main Results:

  • Identified two parallel, cell-nonautonomous positive-feedback loops: Rlm1-Slt2 in feeder cells and Rim101-Ime1 in meiotic cells.
  • The Rlm1-Slt2 loop is activated first, subsequently triggering the Rim101-Ime1 loop via a cell-nonautonomous mechanism.
  • Each loop reinforces its specific cell fate while inhibiting alternative fates, and the second loop represses the first.

Conclusions:

  • Linked cell-nonautonomous positive-feedback loops are crucial for yeast colony pattern formation.
  • These loops amplify microenvironmental differences, potentially serving as a general mechanism for pattern development in various organisms.