Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 3, 2026

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

Numerical modeling for in vivo electroporation.

D Semrov1, D Miklavčič

  • 1University of Ljubljana, Ljubljana, Slovenia.

Methods in Molecular Medicine
|March 30, 2011
PubMed
Summary

Electropermeabilization of cell membranes is a threshold process, requiring specific electric field intensities and pulse parameters. Optimizing these parameters is crucial for effective cell membrane permeabilization, particularly in electro-gene transfection, while avoiding cell death.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples.

Bioelectrochemistry (Amsterdam, Netherlands)·2018
Same author

Dynamic finite-element model for efficient modelling of electric currents in electroporated tissue.

Scientific reports·2016
Same author

Magnetic resonance electrical impedance tomography for measuring electrical conductivity during electroporation.

Physiological measurement·2014
Same author

Cytotoxicity and uptake of archaeosomes prepared from Aeropyrum pernix lipids.

Human & experimental toxicology·2013
Same author

Electrochemotherapy: technological advancements for efficient electroporation-based treatment of internal tumors.

Medical & biological engineering & computing·2012
Same author

Current density imaging during tissue electroporation.

Prilozi·2012

Area of Science:

  • Biophysics
  • Cell Biology
  • Biotechnology

Background:

  • Cell plasma membrane electropermeabilization is a critical process in biotechnology.
  • It relies on inducing a transmembrane potential above a specific threshold.
  • This phenomenon is influenced by external electric field characteristics.

Purpose of the Study:

  • To elucidate the threshold nature of cell membrane electropermeabilization.
  • To define the key parameters influencing this process.
  • To highlight the importance of parameter optimization for applications like electro-gene transfection.

Main Methods:

  • Exposure of cells to electric fields.
  • Induction of transmembrane potential.
  • Analysis of cell membrane permeability changes.
  • Varying electric field intensity, pulse duration, number, and shape.

Main Results:

  • Electropermeabilization is a threshold phenomenon dependent on transmembrane potential.
  • An above-threshold electric field intensity is necessary for permeabilization.
  • Permeabilization is characterized by electric field intensity, pulse duration, number, and shape.
  • Thresholds differ for isolated cells versus cells in tissue.
  • Careful selection of electric field intensity is needed to prevent irreversible damage or cell death.

Conclusions:

  • Cell membrane electropermeabilization is a precisely controlled threshold phenomenon.
  • Optimizing electric field parameters is essential for efficient and safe cell permeabilization.
  • Understanding these thresholds is vital for successful electro-gene transfection and other cellular applications.

More Related Videos

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024

Related Experiment Videos

Last Updated: Jun 3, 2026

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds
08:23

High-Throughput Capable Three-Dimensional Tissue Model for Quantification of Electroporation Thresholds

Published on: August 19, 2025

Single Cell Electroporation in vivo within the Intact Developing Brain
13:31

Single Cell Electroporation in vivo within the Intact Developing Brain

Published on: July 11, 2008

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
08:06

Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring

Published on: September 27, 2024