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Updated: Jun 3, 2026
Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
Published on: January 5, 2011
Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic
Patricia Guerrero-Germán1, Rosa Ma Montesinos-Cisneros, D Miguel F Prazeres
1Departamento de Ingeniería Química y Metalurgia, Universidad de Sonora, Hermosillo, Sonora, México. pguerrero@iq.uson.mx
Abstract:
A novel downstream bioprocess was developed to obtain purified plasmid DNA (pDNA) from Escherichia coli ferments. The intermediate recovery and purification of the pDNA in cell lysate was conducted using hollow-fiber tangential filtration and frontal anion-exchange membrane and elution hydrophobic chromatographies. The purity of the solutions of pDNA obtained during each process stage was investigated. The results show that the pDNA solution purity increased 30-fold and more than 99% of RNA in the lysate was removed during the process operations. The combination of membrane operations and hydrophobic interaction chromatography resulted in an efficient way to recover pDNA from cell lysates. A better understanding of membrane-based technology for the purification of pDNA from clarified E. coli lysate was developed in this research.
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