Histochemical study on the bile duct system of normal rats

The bile duct system of normal rats was examined histochemically. Although the apical surface of biliary and glandular epithelial cells was positively stained with Concanavalia ensiformis (Con A), Dolichos biflorus (DBA), Glycine max (SBA), Ulex europeus-I (UEA-I) and Triticumvulgaris (WGA), the cytoplasm did not apparently react with any lectins. Therefore these cells might not play an important role in an active secretion of mucin. On the other hand, the cytoplasm of goblet cells was positively stained with periodic acid-Schiff, high iron diamine, Con A, DBA, Griffonia simplicifolia-II (GS-II), SBA, UEA-I, and WGA. This suggests that mucin secreted from the goblet cells may be acid one with sulfonic terminals.