Selective solid phase extraction of propranolol on multiwell membrane filter plates modified with molecularly...

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Molecularly imprinted polymers (MIPs) are crucial for selective analyte recognition.
• Traditional solid-phase extraction (SPE) methods can be time-consuming and require larger sample volumes.
• Developing efficient and high-throughput sample preparation techniques is essential for clinical analysis.

Purpose of the Study:

• To develop a novel solid-phase extraction device using MIPs for propranolol cleanup.
• To optimize sample processing parameters for MIP-based extraction.
• To evaluate the suitability of the developed method for biological samples.

Main Methods:

• Synthesis of MIPs within 24-well glass fiber membrane filter plates.
• Optimization of parameters including residence time, sample volume, pH, and solvents.
• Development of a protocol for propranolol quantitation in urine and plasma.

Main Results:

• MIP-modified composite membranes were successfully synthesized.
• Optimized parameters demonstrated efficient propranolol cleanup.
• The method is suitable for low-volume biological samples and offers high-throughput analysis.
• Preliminary validation indicates comparable or superior performance to traditional MISPE cartridges.

Conclusions:

• MIP-modified composite membrane filter plates represent a novel and efficient SPE device.
• This method offers advantages in synthesis ease, speed, and high-throughput analysis.
• The developed protocol provides a viable alternative for propranolol quantitation in biological samples.