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Updated: Jun 3, 2026

13:19
Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
LC-MS based quantification of 2'-ribosylated nucleosides Ar(p) and Gr(p) in tRNA
David Pearson1, Antje Hienzsch, Mirko Wagner
1Center for Integrated Protein Science (CiPSM) at the Department of Chemistry, LMU Munich, Butenandtstrasse 5-13, 81377 Munich, Germany.
Abstract:
RNA nucleosides are often naturally modified into complex non-canonical structures with key biological functions. Here we report LC-MS quantification of the Ar(p) and Gr(p) 2'-ribosylated nucleosides in tRNA using deuterium labelled standards, and the first detection of Gr(p) in complex fungi.
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