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An ultrasonication-assisted extraction and derivatization protocol for GC/TOFMS-based metabolite profiling

Yumin Liu1, Tianlu Chen, Yunpin Qiu

  • 1School of Pharmacy, Center for Instrumental Analysis, Shanghai Jiao Tong University, Shanghai 200240, China.

Analytical and Bioanalytical Chemistry
|March 31, 2011
PubMed
Summary

Ultrasonication drastically cuts metabolite derivatization time to 30 minutes, enhancing efficiency for gas chromatography/time-of-flight mass spectrometry (GC/TOFMS). This improved method identified hypertension biomarkers in human serum, aiding large-scale metabolomic studies.

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