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Protease probes built from DNA: multispectral fluorescent DNA-peptide conjugates as caspase chemosensors

Nan Dai1, Jia Guo, Yin Nah Teo

  • 1Department of Chemistry, Stanford University, Stanford, CA 94305, USA.

Angewandte Chemie (International Ed. in English)
|April 2, 2011
PubMed
Abstract

No abstract available in PubMed .

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...