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Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Updated: Jun 3, 2026

The Microfluidic Probe: Operation and Use for Localized Surface Processing

The Microfluidic Probe: Operation and Use for Localized Surface Processing

Published on: June 4, 2009

Note: A scanning thermal probe microscope that operates in liquids.

Lionel Aigouy¹, Loïc Lalouat, Michel Mortier

¹Laboratoire de Physique et d'Etude des Matériaux, UMR CNRS 8213, ESPCI, 10 rue Vauquelin, 75231 Paris Cedex 5, France. lionel.aigouy@espci.fr

The Review of Scientific Instruments

|April 5, 2011

Summary

This summary is machine-generated.

We created a new scanning thermal probe microscope for liquid environments. This tool uses a fluorescent particle on a tungsten tip to map temperature with high sensitivity, requiring no electrical wiring.

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The Microfluidic Probe: Operation and Use for Localized Surface Processing

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Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy

Studying Dynamic Processes of Nano-sized Objects in Liquid using Scanning Transmission Electron Microscopy

Published on: February 5, 2017

Area of Science:

Microscopy
Thermal Analysis
Nanotechnology

Background:

Accurate temperature mapping in liquids is crucial for understanding various physical and biological processes.
Existing thermal microscopy techniques often face limitations in liquid environments, such as complex probe design and electrical interference.

Purpose of the Study:

To develop a novel scanning thermal probe microscope capable of operating effectively in liquid media.
To demonstrate a new method for non-contact, high-sensitivity temperature measurement at the microscale.

Main Methods:

Utilized a sharp tungsten tip with a fluorescent particle as the thermal sensor.
Employed fluorescence variations of the particle, a thermally sensitive effect, to determine local temperature.
Integrated the thermal probe with scanning microscopy to acquire topographical and thermal images simultaneously.
Operated the microscope in a water/glycerol solution to test its performance in a liquid environment.

Main Results:

Successfully developed a scanning thermal probe microscope functional in liquid environments.
Achieved non-contact temperature determination by monitoring fluorescence changes of the sensing particle.
Obtained simultaneous topographical and thermal images with good sensitivity.
Demonstrated the capability by mapping the temperature of a Joule-heated microheater in a liquid solution.

Conclusions:

The developed scanning thermal probe microscope offers a sensitive and versatile tool for microscale thermal analysis in liquids.
The fluorescent particle-based sensing mechanism eliminates the need for electrical wiring, simplifying probe design and operation.
This technology has potential applications in fields requiring precise thermal characterization of microstructures in fluidic environments.