Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Red Algae01:23

Red Algae

Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
Osmoregulation in Fishes02:32

Osmoregulation in Fishes

When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments?
Fixed Action Patterns01:06

Fixed Action Patterns

A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Other Algae01:19

Other Algae

The group Stramenopiles include some phototrophic microorganisms. Members of this group possess flagella covered in numerous short, hairlike extensions, a feature that inspired the group's name, derived from the Latin words for "straw" and "hair." Some of the main categories of Stramenopiles include diatoms, golden algae, and brown algae.Diatoms are unicellular, photosynthetic eukaryotes, with over 200 known genera. They play a key role in the planktonic communities of both marine and...
Types of Selection01:46

Types of Selection

Natural selection influences the frequencies of particular alleles and phenotypes within populations in several different ways. Primarily, natural selection can be directional, stabilizing, or disruptive. Directional selection favors one extreme trait and shifts the population towards that phenotype while selecting against individuals displaying alternate traits. Stabilizing selection favors an intermediate trait with a narrow range of variation. Deviation from the optimal phenotype towards an...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical Utility of Copy Number Abnormality Analysis in the Evaluation of Melanocytic Lesions for Diagnosis and Prognosis: An Evidence-Based Review from the Cancer Genomics Consortium Working Group for Melanocytic Lesions.

Genes·2026
Same author

Artificial Intelligence-Powered Answers to Questions in Genomic Medicine.

Medical science educator·2026
Same author

Dr. Bradford D. Loucas (1957-2025).

Radiation research·2025
Same author

Distinctive genomic features of hydroa vacciniforme lymphoproliferative disorder in Latin American patients: A multicenter multi-omics study.

Journal of the American Academy of Dermatology·2025
Same author

The Impact of Dose, Sex, and Age at Time of Acute Whole-body Radiation Exposure on Long-term Hematopoiesis in Rhesus Macaques (Macaca mulatta).

Radiation research·2025
Same author

NGS Detects Extensive Genomic Alterations in Survivors of Irradiated Normal Human Fibroblast Cells.

Radiation research·2024
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jun 3, 2026

DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

CO-FISH, COD-FISH, ReD-FISH, SKY-FISH.

Eli S Williams1, Michael N Cornforth, Edwin H Goodwin

  • 1Department of Environmental & Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 5, 2011
PubMed
Summary
This summary is machine-generated.

Chromosome orientation FISH (CO-FISH) reveals DNA sequence orientation and direction within chromosomes. This advanced technique, using telomeres as a reference, has expanded applications in genomic research.

More Related Videos

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies
10:50

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies

Published on: November 8, 2018

Related Experiment Videos

Last Updated: Jun 3, 2026

DNA-based Fish Species Identification Protocol
09:15

DNA-based Fish Species Identification Protocol

Published on: April 28, 2010

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies
10:50

Using the FishSim Animation Toolchain to Investigate Fish Behavior: A Case Study on Mate-Choice Copying In Sailfin Mollies

Published on: November 8, 2018

Area of Science:

  • Genomics
  • Molecular Biology
  • Cytogenetics

Background:

  • Fluorescence in situ hybridization (FISH) is crucial for analyzing chromosome structure and DNA sequence localization.
  • Chromosome orientation FISH (CO-FISH) builds upon standard FISH to determine the relative orientation of DNA sequences within chromosomes.
  • CO-FISH, particularly when using telomeres as a reference, can establish the absolute 5'-3' direction of DNA sequences.

Purpose of the Study:

  • To detail the principles and applications of Chromosome orientation FISH (CO-FISH) and its variation, COD-FISH.
  • To highlight the utility of telomeric DNA as an absolute reference probe for determining DNA sequence orientation.
  • To showcase the evolution and sophisticated applications of CO-FISH in genomic research.

Main Methods:

  • Utilizing CO-FISH to analyze chromosomal rearrangements like inversions and isochromosome formation.
  • Employing telomeric DNA as a reference probe to determine the 5'-3' direction of specific DNA sequences.
  • Integrating CO-FISH with other techniques such as spectral karyotyping (SKY-CO-FISH) for comprehensive genomic analysis.

Main Results:

  • CO-FISH has been instrumental in identifying chromosomal inversions and confirming centromeric asymmetry.
  • Advanced applications include distinguishing telomere synthesis pathways and detecting interstitial telomere sequences.
  • CO-FISH has revealed insights into telomere replication timing, mitotic recombination, and sister chromatid exchange at telomeres.

Conclusions:

  • CO-FISH is a versatile and powerful technique for high-resolution genomic analysis.
  • The method provides critical information on DNA sequence orientation and directionality within chromosomes.
  • CO-FISH continues to evolve, enabling new discoveries in chromosome structure, replication, and stability.