The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme

Area of Science:

• Biotechnology
• Nanotechnology
• Analytical Chemistry

Background:

• Lysozyme is a crucial enzyme in biological systems.
• Accurate lysozyme quantification is vital for diagnostics.
• Existing assays may lack simplicity or sensitivity.

Purpose of the Study:

• To develop a novel, sensitive, and simple assay for lysozyme detection.
• To utilize DNA aptamers and gold nanoparticles for enhanced detection.
• To enable both instrumental and naked-eye lysozyme quantification.

Main Methods:

• Developed a lysozyme DNA aptamer-stabilized gold nanoparticle (AuNP) system.
• Utilized plasmon resonance light scattering (PRLS) for signal transduction.
• Investigated colorimetric changes and PRLS enhancement upon lysozyme binding.

Main Results:

• The assay demonstrated high sensitivity, with a limit of detection around 0.1 nM lysozyme.
• A distinct red-to-blue color change was observed for visual detection at 1 nM lysozyme.
• Linear detection ranges were established for varying AuNP concentrations.
• The method showed good recovery rates in simulated saliva and urine samples.

Conclusions:

• The developed assay is simple, sensitive, and suitable for routine lysozyme analysis.
• The use of DNA aptamers and AuNPs offers a robust platform for biosensing.
• This method holds promise for the analysis of lysozyme in clinical samples.