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Updated: Jun 3, 2026
Corneal Donor Tissue Preparation for Descemet's Membrane Endothelial Keratoplasty
Published on: September 17, 2014
Blunt cannula for descemetic deep anterior lamellar keratoplasty
Vincenzo Sarnicola1, Patricia Toro
1Department of Ophthalmology, Misericordia Hospital, Universita degli Studi di Siena, Grosseto, Italy. v.sarnicola@hotmail.it
Purpose:
To evaluate the success of using a cannula for descemetic deep anterior lamellar keratoplasty (dDALK) and the frequency of Descemet perforation using the new Sarnicola deep anterior lamellar keratoplasty (DALK) cannula and spatula.
Methods:
Retrospective study of medical records of 28 eyes of 28 patients who underwent the DALK procedure between December 2008 and February 2009 using the blunt Sarnicola spatula and cannula. Main outcomes analyzed were the frequency of dDALK and pre-dDALK, the frequency of big-bubble and air-visco bubble formation, and the frequency of Descemet rupture during the procedure.
Results:
In 26 cases (93%), we achieved dDALK dissection; 2 cases (7%) were pre-dDALK. Big-bubble formation occurred in 24 cases (86%); in 2 cases (7%), an air-visco bubble was formed. No perforations of Descemet membrane occurred during air injection in an attempt to form a big bubble. A rupture of Descemet membrane occurred in 1 case (3.5%) during peripheral stromal excision.
Conclusions:
The smooth Sarnicola DALK spatula and cannula facilitate a high percentage of dDALK and make the maneuver more manageable compared to air injection with a needle.
