Adenosine triphosphatase in yeast phase Paracoccidioides brasiliensis

We have previously detected various enzymatic activities in P. brasiliensis. In the present study we have examined Adenosine Triphosphatase (ATPase) activity in yeast phase cultures of P. brasiliensis of increasing age. We employed Wachstein and Meisel's method and electron microscopy and found specific electron-dense deposits indicating ATPase activity to be present in the cytoplasm around vacuoles. Their distribution varied according to age. Deposits decreased or became absent in old cultures. We assume that in P. brasiliensis, ATPase is involved (as in other systems) with transport of Na+ and K+.