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Related Concept Videos

Atomic Nuclei: Nuclear Spin State Overview01:03

Atomic Nuclei: Nuclear Spin State Overview

NMR-active nuclei have energy levels called 'spin states' that are associated with the orientations of their nuclear magnetic moments. In the absence of a magnetic field, the nuclear magnetic moments are randomly oriented, and the spin states are degenerate. When an external magnetic field is applied, the spin states have only 2 + 1 orientations available to them. A proton with = ½ has two available orientations. Similarly, for a quadrupolar nucleus with a nuclear spin value of one, the...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
Atomic Nuclei: Magnetic Resonance01:05

Atomic Nuclei: Magnetic Resonance

The number of nuclear spins aligned in the lower energy state is slightly greater than those in the higher energy state. In the presence of an external magnetic field, as the spins precess at the Larmor frequency, the excess population results in a net magnetization oriented along the z axis. When a pulse or a short burst of radio waves at the Larmor frequency is applied along the x axis, the coupling of frequencies causes resonance and flips the nuclear spins of the excess population from the...
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Atomic Nuclei: Nuclear Spin01:08

Atomic Nuclei: Nuclear Spin

All atomic particles possess an intrinsic angular momentum, or 'spin'. Electrons, protons, and neutrons each have a spin value of ½, although protons and neutrons in nuclei may have higher half-integer spins owing to energetic factors.
Atomic nuclei have a net nuclear spin, , which can have an integer or half-integer value. In atomic nuclei, the spins of protons are paired against each other but not with neutrons, and vice versa. Consequently, an even number of protons does not contribute to...

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Related Experiment Video

Updated: Jun 3, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

A proton spin diffusion based solid-state NMR approach for structural studies on aligned samples.

Jiadi Xu1, Pieter E S Smith, Ronald Soong

  • 1Biophysics and Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, United States.

The Journal of Physical Chemistry. B
|April 7, 2011
PubMed
Summary
This summary is machine-generated.

Researchers developed a new solid-state NMR method to assign resonances in noncrystalline materials. This technique enables atomic-level structural determination of aligned molecules, advancing biomaterial and liquid crystal studies.

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High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
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High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

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Last Updated: Jun 3, 2026

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy
08:55

High-Temperature and High-Pressure In situ Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy

Published on: October 9, 2020

Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Materials science
  • Biophysics

Background:

  • Imaging non-soluble and noncrystalline materials at atomic resolution is challenging.
  • Solid-state NMR is crucial for studying molecular architecture and function.
  • 2D separated-local-field (SLF) spectroscopy provides high-resolution images but lacks resonance assignment.

Purpose of the Study:

  • To develop a resonance assignment approach for 2D SLF experiments.
  • To enable de novo structural determination of aligned noncrystalline materials.
  • To expand the application of solid-state NMR in materials science and biophysics.

Main Methods:

  • Controlled reintroduction of proton spin diffusion into the 2D proton-evolved-local-field (PELF) pulse sequence.
  • Utilizing spin diffusion to measure long-range heteronuclear dipolar couplings.
  • Applying the method to determine the structure of liquid crystalline materials and magnetically aligned bicelles.

Main Results:

  • A novel resonance assignment approach for 2D SLF NMR was demonstrated.
  • The method allows for the measurement of long-range heteronuclear dipolar couplings.
  • Atomic-level resolution structure of N-(4-methoxybenzylidene)-4-butylaniline was determined.

Conclusions:

  • The new PELF pulse sequence with spin diffusion facilitates resonance assignment in solid-state NMR.
  • This technique enhances structural and dynamical studies of aligned molecules.
  • The method is valuable for investigating functional molecules like liquid crystals and biomaterials.