Related Experiment Video
Updated: Jun 3, 2026

Rapid Glyco-Qualitative Assessment of Recombinant Proteins Using a Fully Automated System
Published on: June 28, 2024
β-galactosidases from jack bean and Streptococcus have different cleaving abilities towards fucose-containing sugars
Tomoharu Takeuchi1, Ken-ichi Sugiura, Kazusa Nishiyama
1Laboratory of Molecular Immunology, Faculty of Pharmaceutical Sciences, Josai University, Keyakidai, Sakado, Japan.
Abstract:
We examined the sugar-cleaving abilities of β-galactosidases from jack bean and Streptococcus towards sugars containing fucose residues, and found that jack bean β-galactosidase has an ability to cleave the β1-3 linkage between galactose (Gal) and fucose (Fuc) residues, but not β1-4 linkage. On the other hand, streptococcal β-galactosidase was found to cleave the linkage in both Galβ1-4Fuc and Galβ1-3Fuc disaccharide units. Such a difference in sugar-cleaving abilities between these 2 β-galactosidases will be useful for structural analysis of glycans, especially those from species belonging to Protostomia, such as Caenorhabditis elegans.
Related Concept Videos
Oligosaccharide Assembly
Multiple sugar molecules that may or may...
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Carbohydrate Metabolism
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in the...
