High-throughput analysis of glycoproteins from plasma

The glycoproteome is a major subproteome of plasma. To identify low-abundance plasma glycoproteins and specific glycoforms as disease biomarkers, high-throughput and reproducible methods for glycoproteomic analysis are needed. A glycoproteomic method, utilising solid-phase extraction of glycopeptides (SPEG) based on chemical immobilization of glycopeptides has been developed. The step-by-step protocol for this high-throughput SPEG method using multichannel liquid handling system is described. The protocol provides efficient enrichment for glycoproteins for proteomic identification using mass spectrometry.